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Neutrophil

Neutrophils, or polymorphonuclear (PMN) leukocytes, are highly motile, phagocytic white blood cells that migrate from the blood to sites of tissue damage and infection. Each cell has a distinctive, 3-5 lobed nucleus. Neutrophils, along with eosinophils and basophils, are classified as granular leukocytes because of their characteristic cytoplasmic granules. Neutrophil granules are not visible with routine hematoxylin and eosin staining. 1000x, 6,000x

Neutrophil in circulation > <p>The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.</p>

Neutrophil in circulation >

The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.

 - Neutrophil <p>The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.</p>

- Neutrophil

The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.

 - Barr body <p>The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.</p>

- Barr body

The multiple lobes of this neutrophil nucleus, connected by thin chromatin strands, are clearly evident in this blood smear. Granules are present in the cytoplasm but they are not revealed by this stain. This nucleus also shows a distinctive Barr body, which represents the inactivated second X chromosome in the female. The Barr body appears as a “drumstick-shaped” appendage of the nucleus.

Neutrophils in tissue > <p>Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.</p>

Neutrophils in tissue >

Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.

 - Neutrophils <p>Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.</p>

- Neutrophils

Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.

 - Barr body <p>Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.</p>

- Barr body

Neutrophils are frequently encountered in connective tissues, where they form the first line of defense. They are recruited from the blood stream in response to chemotactic factors released at sites of infection or tissue damage. Neutrophils are phagocytic and remove pathogens and cellular debris. Accumulation of dead neutrophils, bacteria and tissue debris form the whitish-yellow exudate called pus.

Electron microscopy > <p>This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.</p>

Electron microscopy >

This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.

 - Cytoplasmic granules  <p>This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.</p>

- Cytoplasmic granules

This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.

 - Nuclear lobes <p>This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.</p>

- Nuclear lobes

This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.

 - Golgi <p>This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.</p>

- Golgi

This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.

 - Mitochondria <p>This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.</p>

- Mitochondria

This electron micrograph reveals the neutrophil granules. There are two types of granules, azurophilic (primary) granules that contain myeloperoxidase and specific (secondary) granules that are more numerous and contain a variety of antimicrobial enzymes. It is often difficult to distinguish the granule types without special histochemical stains.