Spermatic cord
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
Epithelium
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
Lamina propria
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
Inner longitudinal muscle
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
Middle circular muscle
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
Outer longitudinal muscle
The ductus deferens is lined with pseudostratified epithelium overlying a thin lamina propria. The most diagnostic feature, however, is its thick muscular coat of inner and outer tunics of longitudinally oriented smooth muscle and a middle coat of circular muscle. The differentiation between the green connective tissue and red smooth muscle is readily obvious with this trichrome stain. 40x
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