
Osmium
Osmium tetroxide is both a stain and a fixative for lipid. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Toluidine blue stain of oviduct 1000x

Lipid droplets
Osmium tetroxide is both a stain and a fixative for lipid. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Toluidine blue stain of oviduct 1000x

Nuclei
Osmium tetroxide is both a stain and a fixative for lipid. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Toluidine blue stain of oviduct 1000x

Cytoplasm
Osmium tetroxide is both a stain and a fixative for lipid. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Toluidine blue stain of oviduct 1000x

Cilia
Osmium tetroxide is both a stain and a fixative for lipid. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Toluidine blue stain of oviduct 1000x