Cresyl violet-Luxol fast blue

This combination stain is also called the Kluver-Barrera method and is commonly used to stain nervous tissue. The stain consists of cresyl violet, which stains basophilic structures similarly to hematoxylin, and luxol fast blue, which stains the myelin sheath turquoise. Brainstem 400x

This combination stain is also called the Kluver-Barrera method and is commonly used to stain nervous tissue. The stain consists of cresyl violet, which stains basophilic structures similarly to hematoxylin, and luxol fast blue, which stains the myelin sheath turquoise. Brainstem 400x

This combination stain is also called the Kluver-Barrera method and is commonly used to stain nervous tissue. The stain consists of cresyl violet, which stains basophilic structures similarly to hematoxylin, and luxol fast blue, which stains the myelin sheath turquoise. Brainstem 400x

Nissl substance is the name given to the unique association of rough endoplasmic reticulum and polysomes in neurons.  While both these structures are present individually in most cells, they form these unique, basophilic complexes only in neurons.